Table I. Proteins identified from 293T cells after tandem affinity purification using HA-FLAG-TIN2 epitope tag.
Proteins with peptide ion currents that increased in samples from cells overexpressing the HA-FLAG-TIN2 protein are shown.
| Protein number | Gi codes | Gene symbolsa | Protein descriptors |
|---|---|---|---|
| 1 | 6581060 | TINF2/TIN2 | TERF1 (TRF1)-interacting nuclear factor 2 |
| 2 | 52627149 | TERF2IP/RAP1 | Telomeric repeat binding factor 2, interacting protein |
| 3 | 16877291 | ACD/TPP1 | Adrenocortical dysplasia homolog |
| 4 | 5032169 | TERF2/TRF2 | Telomeric repeat binding factor 2 |
| 5 | 7022355 | POT1 | Protection of telomeres |
| 6 | 2507149 | TERF1/TRF1 | Telomeric repeat binding factor (NIMA-interacting) |
| 7 | 14141152 | HNRPM | Heterogeneous nuclear ribonucleoprotein M |
| 8 | 133254 | HNRPA1 | Heterogeneous nuclear ribonucleoprotein A1 |
| 9 | 306875 | HNRPC | Heterogeneous nuclear ribonucleoprotein C |
| 10 | 5031753 | HNRPH1 | Heterogeneous nuclear ribonucleoprotein H1 |
| 11 | 13384620 | HNRPK | Heterogeneous nuclear ribonucleoprotein K |
| 12 | 32358 | HNRPU | Heterogeneous nuclear ribonucleoprotein U |
| 13 | 4504447 | HNRPA2B1 | Heterogeneous nuclear ribonucleoprotein A2/B1 |
| 14 | 337451 | HNRPAB | Heterogeneous nuclear ribonucleoprotein A/B |
| 15 | 109124618 | HNRPL | Heterogeneous nuclear ribonucleoprotein L |
| 16 | 5031755 | HNRPR | Heterogeneous nuclear ribonucleoprotein R |
| 17 | 4826760 | HNRPF | Heterogeneous nuclear ribonucleoprotein F |
| 18 | 542850 | HNRPG | Heterogeneous nuclear ribonucleoprotein G |
| 19 | 5902102 | SNRPD1 | Small nuclear ribonucleoprotein D1 |
| 20 | 4759160 | SNRPD3 | Small nuclear ribonucleoprotein D3 |
| 21 | 52114 | ELAVL1 | HuR RNA-binding protein |
| 22 | 678271 | TARDBP | TAR DNA-binding protein |
| 23 | 4504259 | HIST1H2BL | Histone cluster 1, H2b1 |
| 24 | 4504301 | HIST1H4A | Histone cluster 1, H4a |
| 25 | 4885373 | HIST1H1A | Histone cluster 1, H1a |
| 26 | 4504251 | HIST2H2AA3 | Histone cluster 2, H2aa3 |
| 27 | 189306 | NCL | Nucleolin |
| 28 | 21626466 | MATR3 | Matrin 3 |
| 29 | 825671 | NPM1 | Nucleophosmin 1 |
| 30 | 5803225 | YWHAE | Tyr 3-monooxygenase/Trp 5-monooxygenase ε |
| 31 | 2580550 | DDX3X | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 |
| 32 | 100913206 | DHX9 | DEAH (Asp-Glu-Ala-Asp) box polypeptide 9 |
| 33 | 38201710 | DDX17 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 |
| 34 | 66346685 | SERBP1 | SERPINE1 mRNA-binding protein 1 |
| 35 | 4826998 | SFPQ | Splicing factor proline/glutamine-rich |
| 36 | 47604944 | SCYL2 | SCY1-like 2 |
| 37 | 52632381 | MAGED1 | NRAGE |
| 38 | 62896605 | EEF1A1 | Eukaryotic translation elongation factor 1 α 1 |
| 39 | 4503483 | EEF2 | Eukaryotic translation elongation factor 2 |
| 40 | 404015 | RPL23A | Ribosomal protein L23a |
| 41 | 550023 | RPS9 | Ribosomal protein S9 |
| 42 | 337930 | RPS4X | Ribosomal protein S4, X-linked |
| 43 | 793843 | RPL29 | Ribosomal protein L29 |
| 44 | 506697 | RPS20 | Ribosomal protein S20 |
| 45 | 5032051 | RPS14 | Ribosomal protein S14 |
| 46 | 2088834 | RPSL28 | Ribosomal protein L28 |
| 47 | 4505995 | PPM1B | Protein phosphatase 1B |
| 48 | 5032027 | RBBP4 | Retinoblastoma-binding protein 4 |
| 49 | 4506439 | RBBP7 | Retinoblastoma-binding protein 7 |
| 50 | 62898329 | SEC61B | Protein translocation complex |
| 51 | 348239 | NONO | Non-POU domain-containing, octamer-binding |
| 52 | 5031741 | DNAJA2 | DnaJ (Hsp40) homolog, subfamily A, member 2 |
| 53 | 219588 | DNAJA1 | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| 54 | 7706326 | SF2B14 | Splicing factor 3B, 14-kDa subunit |
| 55 | 4758302 | ERH | Enhancer of rudimentary homolog |
| 56 | 4885487 | MLF2 | Myeloid leukemia factor 2 |
| 57 | 5729877 | HSPA1A | Heat shock 70-kDa protein 8 |
| 58 | 4529892 | HSPA1B | Heat shock 70-kDa protein 1B |
| 59 | 31542947 | HSPD1 | Heat shock 60-kDa protein 1 (chaperonin) |
| 60 | 9082289 | HSP90AB1 | Heat shock protein 90-kDa α, class B, member 1 |
| 61 | 61656603 | HSP90AA1 | Heat shock protein 90-kDa α, class A, member 1 |
| 62 | 292059 | HSPA9 | Heat shock 70-kDa protein 9 |
| 63 | 35505 | PKM2 | Pyruvate kinase isozymes M1/M2 |
| 64 | 16877071 | ATP5C1 | ATP synthase |
| 65 | 41872631 | FASN | Fatty-acid synthase |
| 66 | 13491174 | MARCKSL1 | MARCKS-like 1 |
| 67 | 2627129 | UBB | Ubiquitin |
| 68 | 5031877 | LMNB1 | Lamin B1 |
| 69 | 4557553 | EMD | Emerin |
| 70 | 62122917 | FLG2 | Filaggrin family member 2 |
| 71 | 416917 | DSG1 | Desmoglein 1 |
| 72 | 6005854 | PHB2 | Prohibitin-2 |
| 73 | 119354192 | KRT1 | Keratin 1 |
| 74 | 547754 | KRT2 | Keratin 2 |
| 75 | 908801 | KRT82 | Keratin type II |
| 76 | 18999435 | KRT5 | Keratin 5 |
| 77 | 55956899 | KRT9 | Keratin 9 |
| 78 | 40354192 | KRT10 | Keratin 10 |
| 79 | 12803709 | KRT14 | Keratin 14 |
| 80 | 1195531 | KRT16 | Keratin 16 |
| 81 | 4557701 | KRT17 | Keratin 17 |
| 82 | 51921 | DCD | Dermcidin |
| 83 | 57546919 | HRNR | Hornerin |
| 84 | 68563515 | KPRP | Keratinocyte protein-rich protein |
| 85 | 190688 | S100A7 | S100-A7 |
| 86 | 16924319 | ACTG1 | Actin, γ1 |
| 87 | 10835838 | 1FH5-L | Immunoglobulin, chain L |
| 88 | 425518 | AAB28159 | Immunoglobulin, heavy chain |
| 89 | 24158782 | 1KFA-L | Immunoglobulin, chain L |
| 90 | 7106439 | TUBB | Tubulin-β |
| 91 | 34740335 | TUBA1B | Tubulin-α1b |
| 92 | 47115317 | VIM | Vimentin |
a Symbols from Human Genome Organisation (HUGO) Gene Nomenclature Database (51). NIMA, never in mitosis gene a, HuR, Hu antigen R, TAR, transactivation-responsive region, NRAGE, neutrophin receptor MAGE, homologue, POU, Pit-1, Oct1, UNC-86, MARCKS, macrophage myristoylated slsnin-tich C kinase substrate.