Fig. 5.

Workflow of peptide to contig assignment for newt ESTs. Individual sequence tags (EST, 5′ reads) from newt tissue were translated into three possible reading frames to generate an in silico peptide database (left) and aligned into contigs on the nucleotide level (right). In silico-translated peptides were identified via MASCOT search, and resulting peptide groups were compared with aligned contigs. More than 90% of peptide/nucleotide alignments were 1:1 assignments.