Table I. Total number of peptides and proteins from global profiling and number of unique tyrosine modified sites corresponding to three modifications (DOPA, dopaquinone, and nitrotyrosine) identified from mouse heart and brain tissue.
| Number of reversed hits/total number of peptides | FPR | Total number of proteins | Number of reversed hits/total number of modified peptides |
Number of tyrosine modified sites after manual inspection |
|||||
|---|---|---|---|---|---|---|---|---|---|
| DOPA | DQ | NO2a | DOPA | DQ | NO2 | ||||
| % | |||||||||
| Brain | 74/26,572 | 0.6 | 4,656 | 2/131 | 2/70 | 0/4 | 50 | 20 | 2 |
| Heart | 27/9,409 | 0.6 | 1,914 | 1/48 | 2/48 | 1/4 | 14 | 12 | 0 |
a Peptide expectation values (log(e)) of <−2 and <−3 were required for fully or partially tryptic NO2-modified peptides.