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ArgT, overexpressed at 30 °C, was degraded at 37 °C without new protein synthesis. The cultures of 2457T/pProEX-argT were induced to express ArgT at 30 °C for 4 h. Then, chloramphenicol was added to prevent new protein synthesis. After that, the cells were incubated at 30 (left) and 37 °C (right) for another 6 h. CK, the cultures of 2457T/pProEX-argT induced to express at 37 °C. M, protein marker (Fermentas).
