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. 2010 Feb 17;9(6):1209–1220. doi: 10.1074/mcp.M900446-MCP200

Fig. 8.

Fig. 8.

Identification of cleavage site in ArgT. A, GST-ArgT and GST-HtrA were incubated at 37 °C for 2 h, and then the degradation products were separated by 15% SDS-PAGE. The two cleavage fragments (frag1 and frag2) were cut out and digested by trypsin and Glu-C. The resolved digestions were analyzed by MALDI-TOF/TOF. B, the PMF result of frag2 digested by trypsin and the MS/MS result of the target peptide (theoretical m/z 1725.82; sequence, AYANQDLVYSDLAAGR). C, the PMF result of frag2 digested by Glu-C and the MS/MS result of the target peptide (theoretical m/z 2581.22; sequence, AYANQDLVYSDLAAGRLDAALQDE). These two peptides suggest the same cleavage site between position 160 and position 161 (VV↓AY) of ArgT. D, part of the sequence of ArgT. The amino acid sequences of the above two peptides are italicized and underlined, respectively. M, protein marker (Fermentas).