Table II. Known virulence factors identified.

Spot ID	GI	Gene	Synonym	Scorea	Sequence coverage	Matched/searched	Peptide sequence confirmed	Peptide scorea	Molecular weight	pI	CAI	ENcb	GRAVY
					%
112	18462586	phoN2/apy	CP0004	153	45	11/64	VICGAHWQSDVEAGR	81	27,727	5.61	0.255	51.07	−0.34
574	18462543	ospF	CP0010	392	66	14/93	SEIPQMLSANER	70	28,095	7.62	0.144	51.89	−0.45
							NNFNILYNQIR	78
							GDFVGDKFHISIAR	98
							IAPGEYPASDVRPEDWK	59
709	18462546	ospD1	CP0022	175	48	8/22	SINNYGLHPANNK	71	24,850	6.45	0.157	56.56	−0.37
496	18462548	ospC2	CP0063	488	33	19/36	SLNLSIEAPSGAR	25	55,744	6.58	0.195	46.17	−0.54
							HQSNANLSNNTLNIK	97
							LGIGLHLIDFIR	70
							FREFCYNK	34
							VLEYFISNGLVDVNKR	105
475	18462537	sepA	CP0070	528	18	24/42	MGDLRDTQGDAGVWAR	57	146,041	6.1	0.288	48.57	−0.36
							DYSSHSWYAGAEVGYR	144
							AGLGYQFDLLANGETVLQDASGEKR	187
395	18462581	ipaA	CP0125	400	59	22/56	DLNTVAVFPELLR	52	70,080	6.13	0.184	44.93	−0.72
							VYDNSYHENPENDAQSPTSQTNDLLSR	144
303	18462578	ipaD	CP0126	647	68	21/34	IANSINDINEQYLK	95	36,696	5.65	0.196	46.76	−0.51
							YKDKPLYPANNTVSQEQANK	130
							YSNANSIFDNLVK	98
							VLSSTISSCTDTDKLFLHF	119
486	56383093	ipaC	CP0127	699	56	19/33	LSEQVQHDSEIIAR	108	38,838	8.54	0.222	50.6	−0.46
							TSLSSPDISLQDKIDTQR	101
							RTYELNTLSAQQK	57
							TYELNTLSAQQK	79
							ATMETSAVAGNISTSGGR	139
504	18462580	ipaB	CP0128	579	31	16/21	NLEFSDKINTLLSETEGLTR	138	62,190	7.66	0.231	44.3	−0.05
							INQIQTR	56
							NDLALFQSLQESR	97
							KSDEYAAEVR	102
017	18462568	ipgC	CP0129	246	79	12/43	GRIEEAEVFFR	36	17,916	4.58	0.26	48.54	−0.15
							NDYTPVFHTGQCQLR	85
520	18462570	ipgB1	CP0130	189	65	18/43	SAQTSISHTALNEIASSGLR	61	23,783	9.56	0.158	55.78	−0.35
560	18462582	icsB	CP0132	749	65	41/70	GPIRVEDTEHGPILIAQK	109	56,613	9.28	0.2	49.28	−0.54
							NYRLENQK	28
							NSIQHSFTGR	89
							DTEINYLFEKR	89
							YIESDFDLSR	92
558	18462567	ipgD	CP0133	166	33	13/23	KLPLSSLELSYSER	50	60,256	9.42	0.16	46.86	−0.48
716	18462559	mxiD	CP0145	296	28	13/25	YVAQSDTVGSFFER	75	63,148	5.54	0.159	47.84	−0.14
							GEDIVIPGVATVVER	41
							TFYVSLVGER	44
755	18462536	spa15	CP0148	172	52	6/50	SNINLVQLVR	27	15,164	4.38	0.187	43.55	0.30
							VVIKDDYVHDGIVFAEILHEFYQR	79
756	18462530	spa47	CP0149	504	54	22/71	LLTQLSFPNR	67	47,774	5.5	0.167	46.64	−0.07
							QTLIKPTAQFLHTQVGR	88
							GYPVSVFDSLPR	97
							SILDGHIYLSR	83
308	56383102	virA	CP0181	668	67	24/50	IITFGIYSPHETLAEK	115	45,163	5.71	0.178	52.28	−0.22
							DTDFNSVWHDIYR	100
							LNFMPEQR	44
							AYEVSSSILPSHITCNGVGINK	64
							IETSYLVHAGTLPSSEGLR	127
L18	18462521	icsA/virG	CP0182	73	6	5/17	TVGGHNEHNLADR	43	116,572	5.33	0.231	51.2	−0.35
366	18462539	phoN1	CP0190	210	42	8/89	LLTNMIEDAGDLATR	90	27,309	6.9	0.24	52.43	−0.29
							AKDEFANNQK	72
L24	56383114	icsP/sopA	CP0271	396	54	16/68	GWLLNNLDYR	71	35,689	9.08	0.225	51.46	−0.51
							LGLIAGYQESR	80
							IPYIGLTANYR	48
							HENFEFGAELK	71

^a Score greater than 49 is significant for a PMF search, and 21 is significant for an ion MS/MS search.

^b ENc is the effective number of codons, which is a measure of the extent of codon preference in a gene.