Fig. 3.

Comparison of HPr phosphorylation pattern between different M. pneumoniae strains. Section of dual channel images of Flamingo fluorescent dye- (green) and Pro-Q Diamond-stained (red) two-dimensional gels displaying the phosphorylated spot of HPr in the M. pneumoniae wild type and different mutant strains. Proteins were separated on an 18-cm IPG strip with a linear pH gradient of pI 4–7 in the first dimension. Note that the HPr spot represents a multiprotein spot containing HPr and UlaB, whereas only UlaB could be detected in the hprK::Tn mutant (much lesser intensity of the phosphorylation spot).