Table I. Phosphoproteins of M. pneumoniae.
For detailed MS/MS data, see supplemental Tables S3 and S4 and Fig. S3. pS, phosphoserine; pT, phosphothreonine.
| Locus name | Protein name | Protein function | COGa | Phospho spots in two-dimensional gel | Phosphosite | Phosphopeptide sequence |
|---|---|---|---|---|---|---|
| MPN001 | DnaN | DNA polymerase III subunit β | L | 2 | ||
| MPN002 | Xdj1 | DnaJ-like protein | O | 2 | ||
| MPN024b | RpoE | RNA polymerase subunit δ | K | 1 | [Ser-87 or Thr-93] | Ip[SQAMFVT]KEIFEEGYEDLSNK |
| MPN025 | Fba | Fructose-bisphosphate aldolase | G | 2 | ||
| MPN050 | GlpK | Glycerol kinase | C | 2 | ||
| MPN053c | HPr | Phosphocarrier protein | G | 1 | ||
| MPN062 | DeoD | Purine-nucleoside phosphorylase | F | 1 | ||
| MPN063 | DeoC | Deoxyribose-phosphate aldolase | F | 1 | ||
| MPN066 | ManB | Phosphomannomutase/phosphoglucomutase | G | 10 | Ser-149 | (K)YHFDGGVNVTApSHNPK |
| MPN082 | Tkt | Transketolase | G | 3 | ||
| MPN120 | GrpE | Heat-shock protein | O | 1 | ||
| MPN154 | NusA | Transcription elongation factor | K | 2 | Ser-503 | PVVKPKpSVFSITVEADDSK |
| MPN221 | Pth | Peptidyl-tRNA hydrolase | J | 1 | ||
| MPN227 | FusA | Elongation factor G | J | 3 | ||
| MPN256b | MPN256 | Uncharacterized protein | S | 2 | Thr-200 | FIDELDQIpTK |
| MPN261 | TopA | DNA topoisomerase I | L | 1 | Ser-426 | TVApSLMADCKK |
| MPN280 | MPN280 | Ribonuclease J | A | 2 | ||
| MPN295 | MPN295 | Uncharacterized protein | S | 2 | Ser-63 | YGPECEKSFLSLQpSK |
| MPN302 | PfkA | Phosphofructokinase | G | 1 | ||
| MPN303 | Pyk | Pyruvate kinase | G | 1 | ||
| MPN311b | P41 | Adhesin-related protein | M | 1 | Thr-223 | TNNSIQQLEAEIQIPpTTHIK |
| MPN322 | NrdF | Ribonucleoside-diphosphate reductase subunit β | F | |||
| MPN324 | NrdE | Ribonucleoside-diphosphate reductase subunit α | F | 3 | Thr-159, Ser-412 | FQPApTPTFLNAGR, VGNDIpSCNLGSLNIAK |
| MPN331 | Tig | Trigger factor | O | 3 | ||
| MPN349 | MPN349 | Uncharacterized protein | S | 1 | ||
| MPN389 | LplA | Lipoate-protein ligase A | H | 1 | ||
| MPN390 | PdhD | Pyruvate dehydrogenase E3 component | C | 2 | ||
| MPN391 | PdhC | Pyruvate dehydrogenase E2 component | C | 4 | ||
| MPN392 | PdhB | Pyruvate dehydrogenase E1 component subunit β | C | 3 | ||
| MPN393 | PdhA | Pyruvate dehydrogenase E1 component subunit α | C | 3 | Ser-205 | TKLEpSAVSDLSTK |
| MPN394 | Nox | NADH oxidase | H | 1 | ||
| MPN420 | GlpQ | Glycerophosphoryldiester phosphodiesterase | C | 1 | ||
| MPN425 | FtsY | Cell division protein homolog | U | 1 | ||
| MPN428 | Pta | Phosphotransacetylase | C | 4 | ||
| MPN429 | Pgk | Phosphoglycerate kinase | G | 1 | ||
| MPN430 | GapA | Glyceraldehyde-3-phosphate dehydrogenase | G | 4 | Ser-245 | VPVLTGpSIVELCVALEK |
| MPN434 | DnaK | Chaperone protein | O | 2 | ||
| MPN452b | HMW3 | Cytadherence high molecular weight protein 3 | M | 1 | ||
| MPN470 | PepP | Xaa-Pro aminopeptidase | E | 2 | Thr-317 | LLCENAVIpTIEPGIYIPSVGGIR |
| MPN474b | MPN474 | Coiled coil surface protein | M | 1 | Thr-773 | QNNEELpTDKCSNIQNELHDLNR |
| MPN495 | UlaB | Ascorbate-specific phosphotransferase enzyme IIB component | G | 1 | ||
| MPN517 | MPN517 | Reductase homolog | C | 1 | ||
| MPN528 | Ppa | Inorganic pyrophosphatase | P | 1 | ||
| MPN533 | AckA | Acetate kinase | C | 4 | ||
| MPN547 | MPN547 | Dihydroxyacetone kinase | C | 1 | ||
| MPN555 | MPN555 | Uncharacterized protein | S | 2 | ||
| MPN572 | PepA | Cytosol aminopeptidase | E | 3 | Ser-249, Thr-420 | YDMSGAAIVCpSTVLALAK, |
| EGVPLIHCDIASpTASIQDLGQGVLVR | ||||||
| MPN573 | GroL | 60-kDa chaperonin | O | 2 | ||
| MPN574 | GroS | 10-kDa chaperonin | O | 1 | ||
| MPN576 | GlyA | Serine hydroxymethyltransferase | E | 1 | ||
| MPN598 | AtpD | ATP synthase subunit β | C | 2 | ||
| MPN600 | AtpA | ATP synthase subunit α | C | 1 | ||
| MPN606 | Eno | Enolase | G | 4 | ||
| MPN625 | MPN625 | Osmotically inducible protein C-like protein | O | 1 | ||
| MPN627 | EI | Phosphotransferase system enzyme I | G | 2 | ||
| MPN628 | Pgm | Phosphoglycerate mutase | G | 1 | ||
| MPN629 | TpiA | Triose-phosphate isomerase | G | 2 | ||
| MPN636 | Frr | Ribosome-recycling factor | J | 2 | ||
| MPN638 | MPN638 | Putative type I restriction enzyme specificity protein | V | 2 | ||
| MPN665 | Tuf | Elongation factor TU | J | 4 | Thr-383 | EGGRpTVGAGSVTEVLE |
| MPN674 | Ldh | l-Lactate dehydrogenase | C | 2 | ||
| MPN677 | MPN677 | Uncharacterized protein | S | 1 | ||
| MPN688 | Soj | ParA family protein | D | 1 |
a COG, cluster of orthologous groups of proteins; A, RNA processing and modification; C, energy production and conversion; D, cell cycle control, cell division, and chromosome partitioning; E, amino acid transport and metabolism; F, nucleotide transport and metabolism; G, carbohydrate transport and metabolism; H, coenzyme transport and metabolism; J, translation, ribosomal structure, and biogenesis; K, transcription; L, replication, recombination, and repair; M, cell wall, membrane, and envelope biogenesis; O, post-translational modification, protein turnover, and chaperones; P, inorganic ion transport and metabolism; S, function unknown; U, intracellular trafficking, secretion, and vesicular transport; V, defense mechanisms.
b Phosphoproteins only detectable in a prpC::Tn mutant (GPM68).
c HPr (MPN053) not detectable in a hprK::Tn mutant (GPM51).