Fig. 1.

Protein phosphorylation in thylakoids from wt and stt7 C. reinhardtii cells in state 1 and state 2. A, isolated thylakoid membranes from C. reinhardtii wt and stt7 cells in state 1 (I) and state 2 (II) were subjected to SDS-PAGE and immunoblotted using anti-phosphothreonine antibody from Cell Signaling Technology. The identity of the phosphorylated proteins was determined by immunoblotting with antisera from the indicated proteins. B, as a loading control, the same protein extracts were immunoblotted with antisera against CP43, CP29, D2, and LHCII as indicated.