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. 2010 Mar 22;285(22):16951–16957. doi: 10.1074/jbc.M109.078501

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Plasmid description. a, mWASH full-length (mWASH-FL), the mWASH WCA module (318–471 amino acids, mWASH-WCA), and the mWASH WC module, lacking the acidic region (318–462 amino acids, mWASH-WCΔA), were cloned in pCMV-Myc-tag3C, pCDNA3-FLAG, and pCDNA3-HA vectors. The full-length of BLOS2 was cloned in pCDNA3-FLAG and pCDNA3-HA vectors. b, two mWASH-derived constructs lacking the WH2 domain (mWASH-FLΔWH2) or the acidic region (mWASH-FLΔA), respectively, were fused to green fluorescent protein in pGFP-C1 plasmid. B, basic region; PPP, polyproline stretch; WH2, Wiskott-Aldrich homologous domain 2; A, acidic region; CC, coiled-coil region.