FIGURE 2.

Characterization of STAT3-binding sites in the mouse steap4 promoter. A, schematic representation of pGL3-steap4 promoter construct and constructs with mutated STAT3 sites that were generated. B, HepG2 cells were transfected with the various luciferase reporter constructs and treated with IL-6. Luciferase activity was measured and normalized to the total protein concentration in the sample (n = 3). C, schematic representation of the mouse and human steap4 promoters demonstrating conservation of STAT3 site 2 and the C/EBPα-binding site. Mut, mutant.