TABLE 1.

Oligonucleotide primers used in this study

The abbreviations are: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mut, mutant; SDM, site-directed mutagenesis; oligo, oligonucleotide. Bold and underlined sequences highlight specific mutations.

Gene/Fragment location	Method	Forward sequence (5′ to 3′)	Reverse sequence (5′ to 3′)
PAI-1	qRT-PCR	gctgaattcctggagctcag	ctgcgccacctgctgaaaca
GAPDH	qRT-PCR	aacctgccaagtacgatgacatc	gtagcccaggatgcccttga
PAI-1 promoter AP-1 (−154/−147 bp)mut	SDM	cacacacacacatgccttgccaagtcccagagaggg	Ccctctctgggacttggcaaggcatgtgtgtgtgtg
PAI-1 promoter HRE-1 (−158/−151 bp)mut	SDM	cacacacatgtatgcctcagcaagtcccagagaggg	Ccctctctgggacttgctgaggcatacatgtgtgtg
PAI-1 promoter AP-1/HRE1 (−154/−151 bp)mut	SDM	cacacacacacaatgctcagcaagtcccagagaggg	Ccctctctgggacttgctgagcattgtgtgtgtgtg
PAI-1:WT oligo (−163/−143 bp)	EMSA	acacacacatgcctcagcaag	cttgctgaggcatgtgtgtgt
PAI-1:HRE-1/AP-1mut	EMSA	acacacaaaagccttggcaag	cttgccaaggcttttgtgtgt
PAI-1:AP1mut	EMSA	acacacacaagccttggcaag	cttgccaaggcttgtgtgtgt
PAI-1 promoter (−366/−82 bp)	ChIP	ctccaagctgaacactagggg	ctggctctgggagtccgtctg
PAI-1 promoter (−114/+98 bp)	ChIP	ccgctggctgttcagacggac	ctggcggcggctgctgagctg