FIGURE 5.
Effect of mTORC1 inhibition with rapamycin on autophagy in IP3R wild-type and TKO cells. A, wild-type and TKO DT40 cells were incubated with 100 nm rapamycin for the indicated times. Cell-free lysates were immunoblotted for LC3, phosphorylated mTOR, and S6 kinase and total mTOR, and S6 kinase. The immunoblots shown are representative of three experiments. B, is the quantitation of the levels of LC3-II at the 2-h time point with the levels of the untreated wild-type cells used as the reference control (100%).