FIGURE 4.

Overexpression of Cdc25A or Cdc25B but not Cdc25C induces an earlier Cdk1-cyclin B complex assembly and activation. A, immunoprecipitations obtained with anti-cyclin B antibodies from 500 μg of lysates of synchronized U2OS Cdc25A cells were used for WB. The right panel shows protein levels in 50 μg of lysates used for IP, detected by WB with the indicated antibodies. B, kinase assays were performed with immunoprecipitations obtained using anti-cyclin B or anti-Cdk1 antibodies from the same lysates as in A. C and D, similar to A and B, but lysates from synchronized inducible U2OS cells expressing Cdc25B were used. E and F, similar to A and B, but lysates from synchronized inducible U2OS cells expressing Cdc25C were used.