FIGURE 4.

Effect of TM11 Cys substitution mutants on PfENT1 transport function and protein expression. A, [³H]adenosine uptake by Cys substitution mutant expressing oocytes as a percent of uptake by wild-type PfENT1-expressing oocytes. * indicates uptake significantly different from WT by one way ANOVA using Dunnett's multiple comparison post hoc test (p < 0.05). HA indicates constructs with a C-terminal HA epitope tag. Mean ± S.E. of three experiments is plotted. B, Western blot of total cellular proteins from oocytes expressing HA epitope-tagged constructs of WT (I), G392C (II), and G396C (III) PfENT1, and uninjected oocytes (IV) is shown. Blot probed with mouse anti-HA primary antibody and a goat anti-mouse horseradish peroxidase-conjugated IgG secondary antibody was visualized by chemiluminescence.