FIGURE 5.

Effect of a 2-min application of 2 mm MTSEA-biotin on subsequent [³H]adenosine uptake by oocytes expressing the TM11 Cys substitution mutants. A, percent inhibition and S.E. for each mutant is shown. * indicate mutants where MTSEA-biotin application caused significantly greater inhibition than the effect on WT by one way ANOVA using Dunnett's multiple comparison post hoc test (p < 0.05). B, control for the presence of the endogenous Cys at position 395. Black bars, [³H]adenosine uptake by untreated oocytes; white bars, [³H]adenosine uptake by MTSEA-biotin treated oocytes. Note that the double mutant does not affect adenosine uptake (black bars), and the effect of a 2-min application of 2 mm MTSEA-biotin is the same in the double mutant C395S/I397C and in the single mutant I397C (white bars). This experiment, panel B, was performed in oocytes from a single frog.