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. 2010 Mar 24;285(22):17001–17010. doi: 10.1074/jbc.M110.115758

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Effect of a 2-min application of various MTS reagents on subsequent [³H]adenosine uptake by oocytes expressing subsets of the TM11 Cys substitution mutants. The x-axis shows percent inhibition of subsequent [³H]adenosine uptake. Effect of 2 mm application of: A, MMTS; B, MTSEA⁺; C, MTSET⁺; D, MTSES⁻; E, MTS-TMR. F, effects of sequential application of MTSEA-biotin and MTS-TMR on subsequent [³H]adenosine uptake by oocytes expressing the T400C mutant PfENT1. The figure in panel F illustrates the results from one batch of oocytes. Similar results were obtained in a second batch of oocytes from a different frog.