TABLE 1.

Primers used for site-directed mutagenesis and systematic N-terminal deletions

Mutation	Primer sequence
R33QFP	5′-CAAGTGGTCAGCCTTTCTGAGAAG-3′
R33QRP	5′-CTTCTCAGAAAGGCTGACCACTTG-3′
D29A-D32AFP	5′-CACGTACGCTGGGAAGGCCCGAGTGGTCAG-3′
D29A-D32ARP	5′-CTGACCACTCGGGCCTTCCCAGCGTACGTG-3′
K31A-R33AFP	5′-CACGTACGATGGGGCGGACGCAGTGGTCAGC-3′
K31A-R33ARP	5′-GCTGACCACTGCGTCCGCCCCATCGTACGTG-3′
E39AFP	5′-GTGGTCAGCCTTTCTGCGAAGAACTTGAAGCAAGTG-3′
E39ARP	5′-CACTTGCTTCAAGTTCTTCGCAGAAAGGCTGACCAC-3′
K40A-K43AFP	5′-CAGTCTTTCTGAGGCAAACTTGGCGCAAGTGTTGA-3′
K40A-K43ARP	5′-TCAACACTTGCGCCAAGTTTGCCTCAGAAAGACTG-3′
Δ5CASQ2FP	5′-GGAATTCCATATGTTGCCGACGTACGATGGGAAGGACC-3′
Δ10CASQ2FP	5′-GGAATTCCATATGGGTAAGGACCGAGTGGTCAGCCT-3′
Δ15CASQ2FP	5′-GGAATTCCATATGGGTCAGCCTTTCTGAGAAGAACTTG-3′
Δ20CASQ2FP	5′-GGAATTCCATATGAAGAACTTGAAGCAAGTGTTGAAGA-3′
CASQRP	5′-CCGCTCGAGTTCATCGTCATCATCGTCATCATCAC-3′