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. 2010 Mar 22;285(22):16921–16930. doi: 10.1074/jbc.M110.111138

FIGURE 2.

FIGURE 2.

Analysis of the interaction between LVIS553 with the PLVIS553 promoter. A, DNase I footprinting. The electropherogram shows a fragment of the digested probe in the absence (black) or presence (white) of LVIS553 highlighting the protected region. The reaction mixture was treated as described under “Experimental Procedures” using as probe the primers shown under Table 1. The nucleotide sequence protected by LVIS553 is shown in the bottom of panel A and boxed in B. B, analysis of PLVIS553 promoter. Predicted Shine-Dalgarno sequence and −10 and −35 of the PLVIS553 are underlined. The protected regions of both plus and minus strands are indicated in a circled box. IR, inverted repeats; MR, mirror region. C, competition EMSA. Labeled PLVIS553 promoter with 10 nm LVIS553 were mixed with increasing concentrations of unlabeled double-stranded FP-553 (30 bp-sequence identified by DNase I footprinting).