FIGURE 3.
Effect of different small molecules on LVIS553 binding to PLVIS553. EMSA results using 2.5 nm biotin-labeled PLVIS553 and 10 nm LVIS553 with different inducer molecules (A) or with increasing concentrations of novobiocin (B) or coumermycin A1 (C). No protein was added to the first lane. The full binding conditions are described under “Experimental Procedures.” In A, lane 1 shows the migration of the target DNA fragment; lanes 2–7, LVIS553 at 10 nm. Carbamezepine (lane 3), canrenoic acid (lane 4), diazoxide (lane 5), pindolol (lane 6), zomepirac (lane 7), and novobiocin (lane 8) were added at a concentration of 100 μm.