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Isothermal titration calorimetric data for the binding of novobiocin to LVIS553. Heat changes (upper panel) and integrated peak areas (lower panel) for the injection of a series of 4-μl aliquots of 1 mm ligand in a solution of 40 μm protein. Protein solutions at 40 μm in 10 mm HEPES, pH 7.5, and 500 mm NaCl were titrated with 1 mm solution of effector. Experiments were carried out at 30 °C.
