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Analysis of the interaction between LAP and mature TGF-β1. Wild type and mutant LAP variants (boldface type and underlined) were generated by site-directed mutagenesis (A), and relative expression levels in transfected HEK-293T cells were determined by Western blot using the FLAG M2 mAb (B). The ability of wild type (WT) and mutant LAP variants to bind mature TGF-β1 and block TGF-β1 signaling was assessed in an in vitro bioassay (C). SP, signal peptide. Error bars, S.D.
