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. 2010 Mar 26;285(22):17077–17088. doi: 10.1074/jbc.M109.065052

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Carbon monoxide effect on ADP/ATP translocase activity of ANT. The results were obtained using isolated non-synaptic mitochondria treated with 10 μm CO, and ADP/ATP translocation was assessed according to Ref. 29. ADP was added to mitochondria and diffused into the intermembrane space through the voltage-dependent anion channel. Once into the intermembrane space, ADP can be transformed by adenylate kinase (AK) in AMP and ATP or exchanged against ATP by adenine nucleotide translocator (ANT). The values are expressed in relative percentage to control (100%) at 15 min of incubation and are mean ± S.D. (error bars), n = 3. *, p < 0.05 compared with control mitochondria.