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. 2010 Mar 19;285(22):16408–16415. doi: 10.1074/jbc.M109.077636

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Cellular localization of each form of RAET1G protein. A and B, lysates of HT1080 and HeLa cells transfected with RAET1G were digested by Endo H (A) or PNGase (B) and subjected to SDS-PAGE (15% gel) separation and Western blotting with polyclonal anti-ULBP2 antibody (AF1298). C, HT1080 cells transduced with RAET1G were surface-stained with monoclonal anti-RAET1G antibody (anti-RAET1G mAb) and after washing, the surface proteins were lysed, immunoprecipitated, and subjected to SDS-PAGE (15% gel) separation and Western blotting with polyclonal anti-ULBP2 antibody (AF1298).