FIGURE 6.

RAET1G expression in GPI-biosynthetic mutant cells. A, lysates of K562 and K562 class K cell lines transduced with RAET1G, ULBP2, or MICB were subjected to SDS-PAGE (15% gel) separation and Western blotting with polyclonal anti-ULBP2 (AF1298) or anti-MICB (AF1599) antibody. B–G, surface expression level of RAET1G (B and C), ULBP2 (D and E), or MICB (F and G) in K562 (B, D, and F) and K562 class K (C, E, and G) cell lines transduced with RAET1G, ULBP2, or MICB were assessed by FACS using monoclonal anti-RAET1G (anti-RAET1G mAb), anti-ULBP2 (MAb1298), or anti-MICB (MAb1599) antibody in the FL4 channel. Shaded area and dashed line represent isotype controls in untransfected and transduced cells, respectively. Solid black line and bold black line represent each antibody staining in untransfected and transduced cells, respectively.