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. 2010 Apr 6;285(22):16623–16631. doi: 10.1074/jbc.M109.098236

FIGURE 2.

FIGURE 2.

C/EBPβ activates RCAN1–4 expression. A, structure of LAP*, LAP, and LIP, indicating DNA-binding (bZIP), regulatory (RD), and activation (AD) domains. B, C2C12 myoblasts were cotransfected with the RCAN1.4-Luc reporter, and expression plasmids encoding LAP, LAP*, or LIP and constitutively activated Cn. Activity is presented as fold activation over the RCAN1.4-Luc reporter alone. C, co-transfection with increasing concentrations of LAP, LAP*, or LIP expression plasmids was used to assess the dose response of the RCAN1.4-Luc reporter. D, C2C12 myoblasts were transfected with LAP and Cn as indicated. Endogenous Rcan1–4 mRNA levels were quantified and normalized to 18 S RNA transcript levels 24 h later. E, Western blot analysis of endogenous RCAN1–4 protein in C2C12 myoblasts 24 h after transfection with a control vector (−) or one expressing LAP. The RCAN1–1 isoform is provided as a loading control. All transfections were carried out in duplicate in a minimum of three independent experiments. Error bars indicate S.D.