FIGURE 3.

Insulin and IGF-1 regulation of gene expression in nondifferentiated versus differentiated WT cells. WT cells were grown to confluence, and differentiation was induced as described under “Experimental Procedures” for 8 days. Nondifferentiated cells at day 0 (Non Diff) and differentiated cells at day 8 (Diff) were serum starved overnight and stimulated with 10 nm insulin (gray bars) or IGF-1 (black bars) during 30 min or 6 h. Gene expression was measured by real time PCR using specific oligonucleotides. The data were normalized to levels of TBP mRNA. Results are mean ± standard error of the mean from 6 independent experiments for nondifferentiated cells and 3 independent experiments for differentiated adipocytes. * indicates a significant difference compared with the similar treatment in nondifferentiated cells, p value <0.05 by Student's t test.