FIGURE 6.

Insulin and IGF-1 dose response on Egr1, Egr2, and Fos gene expression in DKO cells re-expressing IR, IGF1R, or both. DKO cells re-expressing IR, IGF1R, or both were grown to confluence, serum starved overnight, and stimulated with 1, 10, or 100 nm insulin or IGF-1 during 30 min. Egr1, Egr2, and Fos mRNA levels were measured by real time PCR using specific oligonucleotides. The data were normalized to levels of TBP mRNA. Results are mean ± standard error of the mean from 5 independent experiments.