FIGURE 2.

Stable interarray interactions via H4-V21C to H2A-E64C cross-linking persist at decreased oxidizing potentials. A, differential centrifugation characterization of individual arrays and array mixtures following glutathione treatment of the self-associated arrays at varying ratios of oxidized to reduced glutathione, where total glutathione remained constant at 1.0 mm. Shown is the amount of array absorbance remaining in the supernatant for a representative trial. B, differential centrifugation characterization of individual and mixed arrays following 1:15 ox/red glutathione treatment. The array remaining in the supernatant was analyzed as described for Fig. 1D. C, histone composition of individual and mixed arrays following 1:15 ox/red glutathione treatment. Histone components were analyzed as described for Fig. 1E. WT, wild type.