FIGURE 4.
Multiple, but not all, positions on the H4 tail preferentially cross-link to histone H2A-E64C. A, histone composition of cross-linked arrays (1:15 ox/red glutathione treatment, 6.0 mm MgCl2) containing a cysteine residue at various histone H4 tail locations. Histone components were analyzed as described for Fig. 1E. B, differential centrifugation characterization of arrays analyzed in A. Shown is the amount of array absorbance remaining in the supernatant in the absence of MgCl2 for various H4 arrays alone (left bracket) or combined with arrays containing H2A-E64C histone (right bracket). Analysis was performed as described for Fig. 1D.