FIGURE 5.
Conformational changes in MnmE upon association with GidA in the apo state in the presence of K+ (left panel) and Na+ (right panel). In each panel, the left column shows background corrected dipolar evolution data, F(t), and the right column shows distance distributions obtained by Tikhonov regularization, P(t). All of the plots are normalized by amplitude. The modulation depths in all cases coincided with the labeling efficiencies obtained from the cw EPR measurements. The broken lines in the left columns are fits to the data obtained by Tikhonov regularization.