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Mapping of CFTR-binding sites on FLNa. A, diagram of human FLNa and its recombinant fragments fused to His6 tag. B, association of the CFTR1–25 peptide with the His-tagged FLNa fragments. Biotinylated wild-type or S13F mutant CFTR peptides (1.0 μm) were incubated with the His-tagged FLNa fragments (0.1 μm). The peptides were pulled with streptavidin-coated beads, and bound protein was detected by immunoblotting (IB) using anti-His mAb conjugated with peroxidase.
