FIGURE 2.

Localization of CFTR-binding site on IgFLNa9–16. A, GST immunoblot of CFTR1–25 peptide pulldowns from purified GST-tagged FLNa repeats. Biotinylated wild-type or S13F mutant CFTR peptides (1.0 μm) were incubated with the GST-tagged FLNa fragments (0.1 μm). The peptides were pulled with streptavidin-coated beads, and bound protein was detected by immunoblotting (IB) using anti-GST mAb. B, His-8–15 + 24 and deletion mutant (Δ9, deletion of IgFLNa9) were pulled down with increasing amounts of the CFTR1–25 peptide. Bound protein was detected by immunoblotting using anti-His mAb conjugated with peroxidase. WT, wild type.