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Interaction of FLNa with CFTR in vivo. A, indicated Myc-tagged proteins were expressed in BHK cells. Lysates from these cells were incubated with N-terminal wild type (wt) (1–25) or mutant (1–25 S13F) CFTR peptides. Bound proteins were identified by Western blotting (IB) for Myc. B, indicated Myc-tagged proteins were expressed in BHK cells stably expressing CFTR. CFTR was immunoprecipitated, and samples were analyzed by Western blotting for CFTR or Myc.
