FIGURE 8.

Defects in the CFTR-filamin interaction lower the surface expression of wild-type CFTR. A, BHK cells were co-transfected with the indicated myc-tagged constructs and either HA-tagged wild type or S13Fmutant CFTR. Cell surface CFTR was assessed by labeling with a monoclonal HA antibody followed by Alexa Fluor 594-labeled secondary antibodies. Transfected cells were identified by staining with a myc polyclonal antibody. B, myc-positive cells also containing overexpressed CFTR were assessed for CFTR surface expression with at least 50 transfected cells counted/individual experiment *, p < 0.05 versus vector, n = 3. Error bars, S.D.