Table 1. Summary of IGIP expression by cell type and stimulus.
B cells express low levels of IGIP mRNA under stimulation with Phorbol myristate acetate (1µg/ml) and calcium ionophore (100ng/ml) (PMA/I). DCs express low levels of IGIP mRNA under CD40L stimulation and high levels under VIP stimulation. Monocytes/macrophages express low levels of IGIP mRNA under VIP stimulation. Relative Expression (RE) was determined by densitometry. Data shown are from one experiment out of three with similar results, and are graded by the following scale: − = RE < 0.1; + = 0.1 < RE > 0.5; ++ = RE > 0.5.
| T Cells | B Cells | NK Cells | Mono/Mac | DC | |
|---|---|---|---|---|---|
| No Stim. | − | − | − | − | − |
| PMA/I | − | + | − | − | − |
| CD40L | − | − | − | − | + |
| VIP | − | − | − | + | ++ |