Fig. 2.
A-C and D-F: Expression of neurocan by neural cells derived from embryonic stem cells at differentiated day 4. Astrocytes and neurons are identified by their expression of GFAP and β-III tubulin. Expression of hyaluronan by mouse embryonic stem cells one day after differentiation to oligodendrocytes expressing the O4 sulfatide marker (G-J), and to β-III tubulin-expressing neurons (K-M). Panels A-C show the feeder-independent E14T cell line. Nuclear staining with TO-PRO-3 is shown in blue. Magnification: 160×.