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. 2010 May 11;6(3):252–267. doi: 10.7150/ijbs.6.252

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Figure S1 — A DNA map of the nip mutant PBac{RB}mole02670 and rescue of pBac homozygous flie (A) A piggyBac{RB} element is inserted in an intron of the nip gene. The arrows show the location of primers used for PCR to identify pBac insertion in B&C. (B&C) PCR products obtained from genomic DNA of y w (lane 1), pBac/CyO (lane 2) and pBac/pBac;UAS-nip/TubP-Gal4 (lane 3 in B) or pBac/pBac;UAS-nip^NN/AA/TubP-Gal4 (lane 3 in C) flies. The short band in lane 2 of (B) was too weak to detect in the lane 2 of (C).