Parallel MRM screening for 8 different products from the 2nd-generation platform between acetylene II and azides 1–5, 7, 8 and 13 under 4 types of reaction conditions in the presence of: i) CuI, (ii) bCAII, (iii) both bCAII and inhibitor 17, (iv) PBS only. These MRM traces were obtained from 5 sequential injections (a–e) of SPE pre-processed samples. The arrows indicate the time of each injection.