Table 2.

Summary of the screening results from the 256 in situ click chemistry reactions using the ^nd-generation microfluidic platform including some results from the ^st-generation one. The results on the first row were obtained via the LC-MS with the 1^st-generation platform. For MRM-based hit identification, a threshold of 15% of control is used for a positive result.

^*Peak heights for the MRM responses were expressed as a percentage of the response obtained with Cu^I-catalysis. Hit, modest-hit and no-hit reactions were defined as >15%, 5–15% and <5% of the Cu^I-catalysis response, respectively.