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. Author manuscript; available in PMC: 2010 May 28.
Published in final edited form as: Stem Cells. 2010 Jan;28(1):5–16. doi: 10.1002/stem.254

Figure 2.

Figure 2

Activation of NOTCH2 in glioblastoma (GBM) neurospheres increases tumor growth in vitro. (A): When the active form of NOTCH2 receptor (NICD2) was stably transfected into HSR-GBM1a cells, receptor mRNA levels were elevated. (B): NICD2 protein expression was also increased in NICD2 stably transfected HSR-GBM1 neurospheres detected by Western blot. GAPDH was used as protein loading control. (C): The NOTCH target gene HES1 was also induced in NICD2-transfected GBM neurospheres. (D): Cell growth was significantly increased in NICD2-transfected cells. (E): Transient expression of NICD2 in HSR-GBM1 neurospheres using adenovirus vectors also increased cell growth over 2 days. Abbreviations: Ad, Adenovirus; Beta Gal, β-galactosidase; GAPDH, glyceral-dehyde-3-phosphate dehydrogenase; NICD2, NOTCH2 intracellular domain.