Skip to main content
. 2007 Jul;13(7):1081–1083. doi: 10.3201/eid1307.060861

Table 1. Analytical sensitivity for influenza virus in plasma samples*.

PFU (H5N1) spiked 
in minipools No. positive/
no. tested % Positive
250.0 8/8 100
125.0 8/8 100
62.5 8/8 100
31.3 8/8 100
15.6 7/8 87.5
7.8 8/8 100
3.9 4/8 50
0.0 0/8 0

*Influenza (H5N1) standard was extracted from 9.6 mL of 96 pooled donor samples after centrifugation. Five microliters of 75-µL nucleic acid extract was analyzed. The 95% detection limit was 13.4 PFU/mL; the 50% detection limit was 4.8 PFU/mL.