Table 1. Analytical sensitivity for influenza virus in plasma samples*.
PFU (H5N1) spiked in minipools | No. positive/ no. tested | % Positive |
---|---|---|
250.0 | 8/8 | 100 |
125.0 | 8/8 | 100 |
62.5 | 8/8 | 100 |
31.3 | 8/8 | 100 |
15.6 | 7/8 | 87.5 |
7.8 | 8/8 | 100 |
3.9 | 4/8 | 50 |
0.0 | 0/8 | 0 |
*Influenza (H5N1) standard was extracted from 9.6 mL of 96 pooled donor samples after centrifugation. Five microliters of 75-µL nucleic acid extract was analyzed. The 95% detection limit was 13.4 PFU/mL; the 50% detection limit was 4.8 PFU/mL.