Table 2. Analytical sensitivity for avian influenza (H5N1) virus subtype for plasma samples*.

PFU (H5N1) spiked in minipools	No. positive/ no. tested	% Positive
125.0	8/8	100
62.5	8/8	100
31.3	8/8	100
15.6	8/8	100
7.8	7/8	87.5
3.9	7/8	87.5
1.9	4/8	50
0.9	3/8	37.5
0.0	0/8	0

*Influenza (H5N1) standard was extracted from 9.6 mL of 96 pooled donor samples after centrifugation. Five microliters of 75-µL nucleic acid extract was analyzed. The 95% detection limit was 7.4 PFU/mL; the 50% detection limit was 2.5 PFU/mL.