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Two-dimensional gel electrophoresis of the secreted proteome of P. asymbiotica ATCC43949. Proteins were obtained from supernatants of cultures grown at 28°C and 37°C and separated first by isoelectric point, along a 3-10 pH gradient, and then by mass. The 2D gel identifies several proteins with differential levels of production in these conditions, including S1 and S15 (circled) which are only secreted at 28°C.
