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. 2010 May 12;10:141. doi: 10.1186/1471-2180-10-141

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Copyright ©2010 Jones et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pam localization on bacterial cells. (A) Micrograph of a cross-section from a P. luminescens TT01 colony observed by TEM. Note the presence of an extensively folded extracellular matrix (black arrow) between the bacterial cells (indicated with P). (B) Immunolocalization of Pam using the anti-Pam antibody and a conjugated-gold secondary antibody. Gold particles extensively decorate the fibrillar EPS-like matrix (black arrow). (C) The TT01pam strain shows no anti-Pam antibody signal but the fibrillar matrix is still present. Scale bars are 0.2 μm. (D) Western blot confirming the presence of Pam in preparations of crude EPS. Lane 1: crude EPS extracted from TT01rif, lane 2: EPS from TT01pam and lane 3: purified recombinant Pam.