Figure 5.

Comparison of bacterial attachment to surfaces in presence of insect hemolymph by fluorescence microscopy between TTO1rif and the pam mutant. Cells were grown on glass coverslips at 28°C for 8 hours. Planktonic bacteria were washed off and adherent bacteria were fixed and stained with DAPI. The adherence of TT01pam (B) is presented as a percentage of the data determined for the corresponding parental strain TT01rif (A). Bacterial counts were performed at 60× magnification and the data represent the mean values of 12 fields from triplicate experiments (± St.Dev) (C).