Table 1. NCBI blastx ‘best hits’.
| Gene | B. betularia Accession # | Blast hit accession number | Blast hit species | Blast hit gene name | e-value | positives; gaps |
| aaNAT | GU953216 | NP_001073122.1 | Bombyx mori | arylalkylamine N-acetyltransferase | 7e-112 | 236/261 (90%); 0 |
| Ddc | GU953217 | BAB68545.1 | Mamestra brassicae | dopa decarboxylase | 0.0 | 440/457 (96%); 0 |
| Dhpr | GU953218 | XP_001652256.1 | Aedes aegypti | dihydropteridine reductase | 4e-46 | 121/146 (82%); 0 |
| ebony | GU953219 | BAE43845.2 | Papilio xuthus | ebony | 1e-80 | 343/394 (87%); 1 |
| ferritin LC | GU953220 | AAF44717.1 | Manduca sexta | ferritin | 3e-41 | 168/182 (92%); 0 |
| ferritin HC | GU953221 | AAG41120.1 | Galleria mellonella | 26kDa ferritin subunit | 4e-09 | 65/67 (97%); 0 |
| punch | GU953222 | BAH11149.1 | Bombyx mori | GTP cyclohydrolase I isoform A | 6e-95 | 184/194 (94%); 2 |
| henna | GU953223 | BAE66652.1 | Papilio xuthus | phenylalanine hydroxylase | 8e-92 | 167/172 (97%); 0 |
| Pap1 | GU953224 | AAX18636.1 | Manduca sexta | prophenoloxidase-activating proteinase-1 | 2e-151 | 305/379 (80%); 1 |
| Pap3 | GU953225 | AAX18637.1 | Manduca sexta | prophenoloxidase-activating proteinase-3 | 8e-32 | 85/115 (73%); 2 |
| Ppo1 | GU953226 | NP_001037335.1 | Bombyx mori | phenoloxidase subunit 1 precursor | 0.0 | 424/469 (90%); 0 |
| Ppo2 | GU953227 | ABM65701.1 | Heliothis virescens | prophenoloxidase-2 | 0.0 | 617/690 (89%); 1 |
| tan | GU953228 | XP_001599569.1 | Nasonia vitripennis | N/A (conserved hypothetical protein) | 7e-79 | 233/361 (64%); 11 |
| TH | GU953229 | BAE43824.1 | Papilio xuthus | tyrosine hydroxylase | 0.0 | 538/561 (95%); 1 |
| yellow | GU953231 | NP_001037434.1 | Bombyx mori | yellow-y | 0.0 | 442/532 (83%); 26 |
| yellow2 | GU953230 | NP_001037424.1 | Bombyx mori | yellow2/yellow-fa | 1e-145 | 289/349 (83%); 5 |
Blastx results for the 16 candidate genes in the GenBank database. The hits with the highest e-values are listed. The positives and gaps are listed to provide a context for the e-values because a low e-value does not necessarily reflect low similarity. The ‘Low complexity regions filter’ was disabled for the henna search. The tan ortholog of Nasonia vitripennis is not named, instead the Apis mellifera hit with slightly lower score confirms the identity of this gene (acc. # XP_623115.1; Apis mellifera tan; Expect = 2e-74, Positives = 235/353 (66%), Gaps = 11/353 (3%)). Yellow2 blastx reveals two identical B. mori sequences that have been named differently (NP_001037424.1 = yellow2, ABC96695.1 = yellow-fa).