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. 2010 May 28;5(5):e10884. doi: 10.1371/journal.pone.0010884

Figure 3. Late viral protein synthesis is reduced in p85-deficient cells.

Figure 3

p85-deficient cells were infected with VV at an MOI of 5 for 16 hours and subjected to western analysis with various antibodies. A. Cells were infected with VV strain WR expressing B5-GFP under its own promoter. Filters were probed with GFP (top) or tubulin (bottom) antibodies. B. Cells were infected with VV strain WR expressing F13-GFP. Filters were probed with GFP (top) or tubulin antibodies. C. Cells were infected with VV strain WR for 6 or 16 hours. Filters were probed with P4b/4b or tubulin antibodies. Results are from two or three independent trials.