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. 2010 May 28;5(5):e10884. doi: 10.1371/journal.pone.0010884

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. p85-deficient cells were infected with VV B5-GFP for 16 hours. Cells were fixed and stained with phalloidin to visualize actin (red) and DAPI to visualize DNA (blue). Note that in the p85α^−/− and p85α^−/−β^−/− cells there is a reduction of punctate FITC fluorescence at the cell periphery, which represents virions, as well as a reduction in the number of actin tails. B5 remains in a peri-nuclear location in p85-deficient cells. B. p85-deficient cells were infected as in A and percent B5-GFP cells were quantified. Approximately 400 cells were counted per condition and cells were scored positive if a FITC (GFP) signal could be detected. p85α^−/−β^−/− cells had significantly reduced rates of B5-GFP positive cells. Results are from three independent trials. C. p85-deficient cells form fewer actin tails than p85WT cells. Actin tails were counted for ∼10 cells per condition. Data is from one representative experiment.