Figure 1. HIV PIs block LaA maturation, which is reversed rapidly following PI washout.
(A) Blotted with PreA specific and LaA/C antibodies, extracts from Saos-2 cells treated with vehicle (DMSO), Tip, Nel, Ata, Ind and Lop reveal different levels of PreA accumulation. Percentage of mature LaA is listed under each lane. (B) Immunoblot of extracts from Saos-2 cells treated with Lop followed by washout indicates that half time of LaA maturation is approximately 3 hrs. The processing rate of the overnight 35S Cys/Met labeled LaA following Lop washout is similar. Percentage of mature LaA is listed under each lane. (C) Immunofluorescence microscopy of Saos-2 cells double labeled with the antibodies again PreA and Nup153. PreA is accumulated on the NE after Lop treatment and disappears following Lop washout. All images are taken at the same exposure time. In the merged images, DNA, revealed by staining with Hoechst dye, is shown in blue. Bar, 15µm.